HANABI-PIV Metaphase AutoSpreader is an automation device of chromosome metaphase specimen slide of the best development for the gene analyses such as FISH, CGH, and Gbanding. HANABIPIV Metaphase Auto-Spreader is chromosome metaphase specimen making device that succeeded the structure of chromosome metaphase spread device "HANABI" admitted in the world. Samples number 48 and 96 numbers of slides can be processed automatically. The sample tube and the slide glass are managed by the bar code. Crossing of the chromosome is few, and the spread slide can be easy and surely analyze the chromosome.